Thickness of nuclear lamina in MEFs (mouse embryonic fibroblasts)

Value 14 nm Range: ±2 nm
Organism Mammalian tissue culture cell
Reference Turgay Y et al., (2017). The molecular architecture of lamins in somatic cells. Nature. 2017 Mar 9 543(7644):261-264. doi: 10.1038/nature21382 p.262 right column top paragraphPubMed ID28241138
Method Abstract: "Here [investigators] use cryo-electron tomography to obtain a detailed view of the organization of the lamin meshwork within the lamina. Data analysis of individual lamin filaments resolves a globular-decorated fibre appearance and shows that A- and B-type lamins assemble into tetrameric filaments of 3.5nm thickness."
Comments P.262 left column bottom paragraph: "Owing to their large size (~10–20 μm in diameter), nuclei could be easily identified at low magnification in cryo-EM images, confirming successful sample preparation and preservation of the overall size and shape of nuclei (Extended Data Fig. 2a–c). The reconstructed cryo-tomograms revealed the presence of ~3.5 nm thick filaments forming a complex meshwork at the nuclear periphery, underneath nuclear pore complexes (NPCs) (Fig. 1a, b). These filaments varied in length and displayed a pattern of sparsely and densely packed regions (Fig. 1a), which were also detected above dense nuclear material (presumably chromatin remnants, Extended Data Fig. 2c–e). Statistical analysis of these filaments indicated a length of 380 ± 122 nm (Fig. 1c). The persistence length distribution of these filaments varied between 50 and 2,700 nm (Fig. 1d), indicating a high degree of flexibility, which is in agreement with measurements of the previously analysed Caenorhabditis elegans lamin [ref 16] and the short filaments found at the nuclear periphery in HeLa cells [ref 14]. Furthermore, [investigators] show that these filaments are packaged into a 14 ± 2 nm thick layer (Fig. 1e)."
Entered by L Tal
ID 114285