||Mammalian tissue culture cell
||Tseng Y, Lee JS, Kole TP, Jiang I, Wirtz D. Micro-organization and visco-elasticity of the interphase nucleus revealed by particle nanotracking. J Cell Sci. 2004 Apr 15117(Pt 10):2159-67.PubMed ID15090601
||Through the use of particle nanotracking, researchers detect and quantify the micro-organization as well as the viscoelastic properties of the intranuclear region in single, live, interphase somatic cells. Shear flow experiments-Glass coverslips were cleaned and treated with 0.1-% poly-L-lysine (Sigma, St Louis, MO), and then coated with 20 µg/ml fibronectin (Calbiochem, San Diego, CA).
||The intranuclear region is much stiffer than the cytoplasm. It is also more elastic than viscous, which reveals that the intranuclear region displays an unexpectedly strong solid-like behavior. 1 Poise = 1 g·cm^-1·s^-1.