| Value |
15
min
|
| Organism |
Mouse Mus musculus |
| Reference |
Alpuche-Aranda CM, Racoosin EL, Swanson JA, Miller SI. Salmonella stimulate macrophage macropinocytosis and persist within spacious phagosomes. J Exp Med. 1994 Feb 1 179(2):601-8 link abstract and p.604 right column 2nd paragraph PubMed ID8294870
|
| Primary Source |
[23] Racoosin EL, Swanson JA. Macropinosome maturation and fusion with tubular lysosomes in macrophages. J Cell Biol. 1993 Jun121(5):1011-20PubMed ID8099075
|
| Method |
Abstract: "Light microscopic studies of phagocytosis showed that Salmonella typhimurium entered mouse macrophages enclosed in spacious phagosomes (SP). Viewed by time-lapse video microscopy, bone marrow-derived macrophages exposed to S. typhimurium displayed generalized plasma membrane ruffling and macropinocytosis." Primary source abstract: "Macropinosomes formed by addition of recombinant macrophage colony-stimulating factor (rM-CSF) to mouse macrophages migrate centripetally and shrink, remaining detectable by phase microscopy for up to 15 min." |
| Comments |
Abstract: "In contrast to macropinosomes induced by growth factors, which shrink completely within 15 min, SP [spacious phagosomes] persisted in the cytoplasm, enlarging often by fusion with macropinosomes or other SP." P.604 right column 2nd paragraph: "Time-lapse video microscopy indicated that SP [spacious phagosomes] shrank more slowly than M-CSF [macrophage-colony stimulating factor]-induced macropinosomes, which usually become undetectably small within 10-15 min of formation (primary source). [Investigators] therefore quantified SP and macropinosomes at different times after the addition of gentamicin to culture medium to eliminate extracellular bacteria." |
| Entered by |
Uri M |
| ID |
116146 |