||Mouse Mus musculus
||Palazzo AF, Springer M, Shibata Y, Lee CS, Dias AP, Rapoport TA. The signal sequence coding region promotes nuclear export of mRNA. PLoS Biol. 2007 Dec5(12):e322. DOI: 10.1371/journal.pbio.0050322 p.2864 right columnPubMed ID18052610
|| Audibert A, Weil D, Dautry F. In vivo kinetics of mRNA splicing and transport in mammalian cells. Mol Cell Biol. 2002 Oct22(19):6706-18.  Snaar SP, Verdijk P, Tanke HJ, Dirks RW. Kinetics of HCMV immediate early mRNA expression in stably transfected fibroblasts. J Cell Sci. 2002 Jan 15 115(Pt 2):321-8.PubMed ID12215528, 11839784
||P.2863 right column bottom paragraph: "To monitor the nuclear export of t-ftz-i mRNA [see comments section], transcripts were microinjected into nuclei of NIH 3T3 cells. The cells were fixed at various time points, and the localization of the injected RNA was probed by fluorescence in situ hybridization (FISH). [Investigators] optimized the FISH procedure, omitting harsh acid and ethanol treatments, such that intracellular morphology was largely maintained. [They] estimate that 20,000 to 50,000 transcripts were injected per cell, which is a small number compared with the total number of transcripts in a typical mammalian cell (400,000 to 850,000 molecules) [ref 35 BNID 113016]. Again, fluorescent 70-kDa dextran was co-injected to identify nuclear-injected cells (Figure 2, insets). Immediately after injection, the transcripts were confined to the nucleus. Over time, however, the majority of t-ftz-i transcripts (∼80%) accumulated in the cytoplasm (Figure 2A). Quantitation showed that the half-time of mRNA export was ∼15 min (Figure 2C), similar to previous estimates [primary sources]. About 50% of the mRNA molecules remained intact after 4 h, as shown by the total level of FISH signal (Figure 2D)."
||For explanation of "t-ftz-i mRNA" see p.2863 right column 2nd paragraph link SSCR=signal sequence coding region