Changes in DNA replication produced by inhibitors of protein synthesis in fibroblasts line L-929 in vitro

Range antibiotic added at time of hot pulse 1.91kb/min Table - link kb/min
Organism Mouse Mus musculus
Reference Hand R. Deoxyribonucleic acid fiber autoradiography as a technique for studying the replication of the mammalian chromosome. J Histochem Cytochem. 1975 Jul23(7):475-81. p.478 table IPubMed ID1095649
Method Abstract: "Deoxyribonucleic acid (DNA) fiber autoradiography is a technique that allows analysis of replication events on mammalian chromosomal DNA. 3H-thymidine is used to pulse-label DNA, which is subsequently released from lysed cells and extended linearly over a glass slide. Autoradiograms are produced by exposing the labeled DNA to light-sensitive emulsion. The effects of inhibition of protein synthesis on DNA replication were examined using this technique." See notes beneath table
Comments P.477 left column top paragraph: "In experiments in which cycloheximide was added at the beginning of a 30-min hot pulse, the antibiotic produced a decreased rate of fork progression within the 30 min of the hot pulse. Puromycin did not significantly retard fork progression when added at the time of the hot pulse (Table I, experiment 1)."
Entered by Uri M
ID 112881