Elongation rate of RNA polymerase II for both A20 and Icam1 in fibroblasts

Range ~8 kb/min
Organism Mouse Mus musculus
Reference Hao S, Baltimore D. RNA splicing regulates the temporal order of TNF-induced gene expression. Proc Natl Acad Sci U S A. 2013 Jul 16 110(29):11934-9. doi: 10.1073/pnas.1309990110. p.11937 right column 2nd paragraphPubMed ID23812748
Method Abstract: "When cells are induced to express inflammatory genes by treatment with TNF [Tumor necrosis factor], the mRNAs for the induced genes appear in three distinct waves, defining gene groups I, II, and III, or early, intermediate, and late genes. To examine the basis for these different kinetic classes, [investigators] have developed a PCR-based procedure to distinguish pre-mRNAs from mRNAs."
Comments P.11937 right column 2nd paragraph: "[Investigators] saw an ∼1.6-min lag between the rise of pre-mRNA at exon 2 (J2) of Tnfaip3 and at exon 8 (J8) (a distance of 8.1 kb) (Fig. 5A). These values translate into an average elongation speed of 5 kb/min, which is somewhat faster than reported elsewhere based on in vitro or artificial reporter systems (refs 13, 27–29). A similar high elongation speed (5.2 kb/min) was found for group II gene Icam1, between the 5′ end (J2) and 3′ end (J2′) of intron 2 in macrophages (Fig. 5B). This similarity in elongation speeds clearly indicates that the delayed appearance of Icam1 mRNA is not due to slowness of transcription, at least over the regions that [they] studied. A similar result is also observed in mouse fibroblasts, in which an even higher elongation speed (∼8 kb/min) was reproducibly observed for both A20 and Icam1 (Fig. S5A)."
Entered by Uri M
ID 112441