RNA Pol II transcription elongation rate in ES [Embryonic Stem] cells

Range 0.5 - 4 kb/min
Organism Mouse Mus musculus
Reference Jonkers I, Kwak H, Lis JT. Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons. Elife. 2014 Apr 29 3: e02407. doi: 10.7554/eLife.02407. p.15 top paragraphPubMed ID24843027
Method (Abstract:) "To dissect Pol II dynamics in mouse ES cells, [researchers] inhibited Pol II transcription at either initiation or promoter-proximal pause escape with Triptolide or Flavopiridol, and tracked Pol II kinetically using GRO-seq [global run-on sequencing]...Finally, tracking the progression of Pol II after drug treatment establishes Pol II elongation rates at over 1000 genes."
Comments "[Researchers] measured elongation rates ranging from 0.5 to 4 kb/min at ~1000 genes and within different regions of genes. Strikingly, Pol II accelerates as it transcribes through the gene body, and a large amount of variation in elongation rates occurs between genes, and is associated with chromatin and exon density."
Entered by Uri M
ID 111273