Concentration of most abundant amino acids in mitochondrial matrix of HeLa cell

Range aspartate 1.6mM: alanine 327µM: histidine 82µM
Organism Human Homo sapiens
Reference Chen WW, Freinkman E, Wang T, Birsoy K, Sabatini DM. Absolute Quantification of Matrix Metabolites Reveals the Dynamics of Mitochondrial Metabolism. Cell. 2016 Aug 25 166(5):1324-1337.e11. doi: 10.1016/j.cell.2016.07.040. p.1328 right column 3rd paragraphPubMed ID27565352
Method P.1325 left column top paragraph: "...[investigators] developed a new method that combines rapid immunocapture of epitope-tagged mitochondria with metabolite profiling by liquid chromatography and mass spectrometry (LC/MS)."
Comments P.1328 right column 3rd paragraph: "One particularly diverse class of matrix metabolites is amino acids, which participate in both metabolic reactions and the synthesis of the 13 mitochondrially encoded proteins required for RC [respiratory chain] activity (Elo et al., 2012). The majority of proteinogenic amino acids were found in mitochondria (Figure 2C Tables S1, S2, and S3), with the most abundant being aspartate (1.6 mM), alanine (327 μM), and histidine (82 μM) (Figure 2C). Although the mitochondrial abundance of different amino acids will likely vary among different cell types, the high concentration of aspartate agrees with recent findings demonstrating that a critical role for mitochondria in supporting cell proliferation is aspartate synthesis (Birsoy et al., 2015 and Sullivan et al., 2015)."
Entered by Uri M
ID 112851