Concentration of most abundant metabolites in mitochondrial matrix of HeLa cell

Range aspartate 1.6mM: phosphocholine 1.47mM: GSH (Glutathione) 1.37mM: NAD 818µM
Organism Human Homo sapiens
Reference Chen WW, Freinkman E, Wang T, Birsoy K, Sabatini DM. Absolute Quantification of Matrix Metabolites Reveals the Dynamics of Mitochondrial Metabolism. Cell. 2016 Aug 25 166(5):1324-1337.e11. doi: 10.1016/j.cell.2016.07.040. p.1328 right column 2nd paragraphPubMed ID27565352
Method P.1325 left column top paragraph: "...[investigators] developed a new method that combines rapid immunocapture of epitope-tagged mitochondria with metabolite profiling by liquid chromatography and mass spectrometry (LC/MS)."
Comments P.1328 right column 2nd paragraph: "Concentrations of most metabolites within the mitochondrial matrix were generally lower than the corresponding measurements made at the whole-cell level (Figures 2C and S2B). The most abundant metabolites were aspartate (1.6 mM), phosphocholine (1.47 mM), GSH (1.37 mM), and NAD (818 μM), each of which participate in distinct metabolic processes (Figure 2C). In contrast, the least abundant were methylthioadenosine (31 nM), inosine (26 nM), and urate (17 nM), all metabolites involved in nucleotide metabolism. As a whole, matrix concentrations spanned a wide range of values, even within the same family of metabolites, underscoring the diversity of the metabolic space within mitochondria."
Entered by Uri M
ID 112849