10 - 20 %
||Chen WW, Freinkman E, Wang T, Birsoy K, Sabatini DM. Absolute Quantification of Matrix Metabolites Reveals the Dynamics of Mitochondrial Metabolism. Cell. 2016 Aug 25 166(5):1324-1337.e11. doi: 10.1016/j.cell.2016.07.040. p.1326 left columnPubMed ID27565352
||J.A. Idell-Wenger, L.W. Grotyohann, J.R. Neely, Coenzyme A and carnitine distribution in normal and ischemic hearts, J. Biol. Chem., 253 (1978), pp. 4310–4318 AND J.R. Williamson, B.E. Corkey, Assay of citric acid cycle intermediates and related compounds—Update with tissue metabolite levels and intracellular distribution, Methods in Enzymology, Academic Press (1979), pp. 200–222PubMed ID207696, 459841
||P.1325 left column top paragraph: "...[investigators] developed a new method that combines rapid immunocapture of epitope-tagged mitochondria with metabolite profiling by liquid chromatography and mass spectrometry (LC/MS)."
||P.1326 left column: "Both the enzyme citrate synthase (CS) and the metabolite coenzyme A (CoA) predominantly reside in the mitochondrial matrix, with only 10%–20% of cellular CoA being extra-mitochondrial (primary sources)." 1st primary source studied rat heart