Top 20 most abundant proteins in each of mitochondrion, plasma membrane & endoplasmic reticulum of HeLa cell

Range Figure - link % of protein organelle mass
Organism Human Homo sapiens
Reference Itzhak DN, Tyanova S, Cox J, Borner GH. Global, quantitative and dynamic mapping of protein subcellular localization. Elife. 2016 Jun 9 5. pii: e16950. doi: 10.7554/eLife.16950. p.59 figures 3C-3EPubMed ID27278775
Method Abstract: "[Investigators] have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. [They] initially used maps statically to generate a database with localization and absolute copy number information for over 8,700 proteins from HeLa cells, approaching comprehensive coverage. All major organelles were resolved, with exceptional prediction accuracy (estimated at >92%). Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. [They] subsequently demonstrated the dynamic capabilities of the approach by capturing translocation events following EGF [epidermal growth factor] stimulation, which [they] integrated into a quantitative model."
Comments P.8 bottom paragraph: "Quantitative anatomy of a HeLa cell: Combined knowledge of protein subcellular localization and abundance enables construction of a model of HeLa cell composition. [Investigators] calculated the protein mass of each organelle by multiplying the molecular weights of constituent proteins by their estimated copy numbers (Figure 3)." P.45 2nd paragraph: "Figure 3C-E: Top 20 most abundant proteins in each of the three major organelles, plotted against their contribution to protein organelle mass. The complete quantitative composition of ER, mitochondria, and plasma membrane are shown in Supplementary file 5."
Entered by Uri M
ID 112704