Time for depletion of readily releasable pool (RRP) of synaptic vesicles (SVs) after stimulation at 30 Hz

Range 10 – 15 sec
Organism Frog Rana pipiens
Reference Richards DA, Guatimosim C, Rizzoli SO, Betz WJ. Synaptic vesicle pools at the frog neuromuscular junction. Neuron. 2003 Jul 31 39(3):529-41. p.530 left column bottom paragraphPubMed ID12895425
Method Abstract: "[Investigators] have characterized the morphological and functional properties of the readily releasable pool (RRP) and the reserve pool of synaptic vesicles in frog motor nerve terminals using fluorescence microscopy, electron microscopy, and electrophysiology."
Comments P.530 left column bottom paragraph: "These and other observations suggested that the readily releasable pool is depleted after 10–15 s of stimulation at 30 Hz, and that, if stimulation continues beyond 10–15 s, reserve pool vesicles are mobilized and undergo exocytosis. Thus, [investigators] define the RRP as the fraction of the total vesicle pool that is readily available for release with tetanic stimulation from the standpoint of endocytosis, the RRP is defined as the vesicles that can rapidly recycle without a need for endosomal intermediates. [They] show below that a third definition also identifies this population, namely the vesicles that are available for release with low-frequency stimulation. In the present work, [they] have characterized and quantified functional and morphological properties of these pools using optical, electrophysiological, and electron microscopic techniques."
Entered by Uri M
ID 112565