Half-time of myosin II redistribution to the cell cortex upon LPA [lysophosphatidic acid] stimulation

Range ~20 sec
Organism Zebrafish Danio rerio
Reference Ruprecht V et al., Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 2015 Feb 12 160(4):673-85. doi: 10.1016/j.cell.2015.01.008. p.675 left column 5th paragraphPubMed ID25679761
Method P.675 left column 5th paragraph:"To address the effect of LPA stimulation on stable-bleb cell polarization, [investigators] monitored the distribution of cortical markers in the presence of different LPA concentrations."
Comments P.675 left column 5th paragraph:"Myosin II rapidly redistributed to the cell cortex upon LPA stimulation (t1/2 ∼20 s, Figure 2D), and rising LPA concentrations were associated with increasing cortical accumulations of myosin II, eventually saturating at a threshold level at which progenitors transformed into stable-bleb cells (Figures 2E and 2F). Importantly, the accumulation of myosin II at the cortex was up to seven times larger than the accumulation of actin, indicating that myosin II-mediated cortical contractility was specifically elevated upon LPA stimulation (Figures 2G and S1D)."
Entered by Uri M
ID 112241