Speed with which transformed cells migrated away from the region of transplantation

Value 8.5 μm/min Range: ±0.3 μm/min
Organism Zebrafish Danio rerio
Reference Ruprecht V et al., Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 2015 Feb 12 160(4):673-85. doi: 10.1016/j.cell.2015.01.008. p.683 left column bottom paragraphPubMed ID25679761
Method P.683 left column bottom paragraph:"To test if stable-bleb cells emerge at local wounding sites within the gastrulating embryo, [investigators] transplanted fluorescently labeled donor cells into an unlabeled host embryo at sphere stage (4 hr post fertilization [hpf]) (Figures 7C and 7D). Using Tg(actβ1:myl12.1eGFP) donor embryos, elevated cortical accumulations of myosin II were detected around the wounding area (Movie S8) and, interestingly, labeled donor cells stochastically transformed into stable-bleb like cells with a highly polarized cortical architecture and fast retrograde cortical flows similar to their morphology in vitro (Figure 7E Movie S8)."
Comments P.683 left column bottom paragraph: "Transformed cells migrated away from the region of transplantation with speed (v = 8.5 ± 0.3 μm/min) and directionality much higher than any other cells yet described in the zebrafish embryo (Figures 7F and 7G). Together, this indicates that stable-bleb motility can be induced at places of high contractility within the gastrulating embryo, suggesting a mechanism for rapid cell extrusion from highly contractile regions within the embryo."
Entered by Uri M
ID 112244