| Value |
8.5
μm/min
Range: ±0.3 μm/min
|
| Organism |
Zebrafish Danio rerio |
| Reference |
Ruprecht V et al., Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 2015 Feb 12 160(4):673-85. doi: 10.1016/j.cell.2015.01.008. p.683 left column bottom paragraphPubMed ID25679761
|
| Method |
P.683 left column bottom paragraph:"To test if stable-bleb cells emerge at local wounding sites within the
gastrulating embryo, [investigators] transplanted fluorescently labeled
donor cells into an unlabeled host embryo at sphere stage (4 hr post fertilization [hpf]) (Figures 7C and 7D). Using Tg(actβ1:myl12.1eGFP) donor embryos, elevated cortical accumulations
of myosin II were detected around the wounding area (Movie S8) and, interestingly, labeled donor cells stochastically transformed into stable-bleb like cells with a highly
polarized cortical architecture and fast retrograde cortical flows similar to their morphology in vitro (Figure 7E Movie S8)." |
| Comments |
P.683 left column bottom paragraph: "Transformed cells migrated away from the region of transplantation with speed (v = 8.5 ± 0.3 μm/min) and directionality much higher than any other cells yet described in the zebrafish embryo (Figures 7F and 7G). Together, this indicates that stable-bleb motility can be induced at places of high contractility within the gastrulating embryo, suggesting a mechanism for rapid cell extrusion from highly contractile regions within the embryo." |
| Entered by |
Uri M |
| ID |
112244 |