Average speed of stable-bleb cells plated on fibronectin-coated substrates under confinement

Value 17.1 µm/min Range: ±0.6 µm/min
Organism Zebrafish Danio rerio
Reference Ruprecht V et al., Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 2015 Feb 12 160(4):673-85. doi: 10.1016/j.cell.2015.01.008. p.679 right columnPubMed ID25679761
Method P.677 right column bottom paragraph:"To study the migratory behavior of polarized stable-bleb cells, [investigators] monitored their motility in different environments (Figure 5A). Stable-bleb cells only weakly adhered to and failed to migrate on 2D adhesive substrates coated with ECM [Extracellular matrix] components, such as Laminin and Fibronectin. Fluorescence imaging of the subcellular distribution of molecules associated with coupling the cortex to the surrounding substrate, such as N-Cadherin, Ezrin, and Integrin revealed a highly asymmetric accumulation toward the cell rear, while the bleb-like protrusion front was largely devoid of those molecules (Figures 5B and 5C). This indicates only weak adhesive forces in the front that are insufficient for forward locomotion on 2D substrates. To increase frictional coupling of migrating cells, [they] thus created a planar 2D confinement by placing cells between a coated glass substrate and an agarose layer."
Comments P.679 right column:"[Investigators] found that stable-bleb cells plated on Fibronectin-coated substrates under confinement displayed exceptionally fast and directional movements with average cell speed <vcell> = 17.1 ± 0.6 μm/min (n = 67 cells) and persistence length of approximately four cell diameters (Figures 5E and 5F), best described by a persistent random walk model over long timescales (Figure 5F)."
Entered by Uri M
ID 112242