Speed of progenitor cells induced to be of mesodermal or mesendodermal origin and placed on fibronectin-coated substrates

Value 3.8 µm/min Range: ±0.3 µm/min
Organism Zebrafish Danio rerio
Reference Ruprecht V et al., Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 2015 Feb 12 160(4):673-85. doi: 10.1016/j.cell.2015.01.008. p.674 left column top paragraphPubMed ID25679761
Method P.673 right column bottom paragraph:"To study the emergence of migration competence in early germ layer progenitor cells, [investigators] aimed at developing in vitro assays to investigate the complex range of migration behaviors observed in vivo under controlled conditions with a minimal set of defined environmental parameters."
Comments P.674 left column top paragraph:"Early progenitor cells placed on 2D substrates displayed a characteristic blebbing morphology that can also be observed in early blastula stage embryos in vivo (Diz-Muñoz et al., 2010). Notably, those blebbing cells failed to migrate irrespective of adhesive substrate coating with extracellular matrix (ECM) components, such as Laminin or Fibronectin (Figure 1A Movie S1 available online). However, when progenitor cells were induced to be of mesodermal or mesendodermal origin and placed on Fibronectin-coated substrates, they formed a characteristic mixture of lamellipodia and filopodia (Figure 1B) and underwent collective migration with similar speed (<vCell> = 3.8 ± 0.3 μm/min) to their movement in vivo (Figure 1B′ Movie S1)."
Entered by Uri M
ID 112240