| Range |
~165 bp
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Michael Lynch and Georgi K. Marinov, The bioenergetic costs of a gene, PNAS 2015 doi: 10.1073/pnas.1514974112 link Supplementary Materials p.1 right column bottom paragraph |
| Primary Source |
[20] Smith DJ, Whitehouse I. 2012. Intrinsic coupling of lagging-strand synthesis to chromatin assembly. Nature 483(7390):434-438. doi: 10.1038/nature10895.PubMed ID22419157
|
| Method |
Primary source abstract:"Using deep sequencing, [investigators] demonstrate that ligation junctions preferentially occur near nucleosome midpoints rather than in internucleosomal linker regions." |
| Comments |
Supplementary Materials p.1 right column bottom paragraph:"But because Okazaki fragments in eukaryotes appear to be short - averaging ~165 bp in yeast [primary source], with similar values in human and other eukaryotes [refs 21-23], there are again generally large numbers of Okazaki fragments per inter-ORI [origin of replication] interval, so the investment in RNA primers and ligation on the leading strand is minor relative to that on the lagging strand and can be ignored." |
| Entered by |
Uri M |
| ID |
112149 |