Length of Okazaki fragment

Range Escherichia coli 1,000 - 2,000: Pyrococcus abyssi ~100 nts
Organism prokaryote
Reference Rocha EP. The replication-related organization of bacterial genomes. Microbiology. 2004 Jun150(Pt 6):1609-27. p.1611 left column top paragraphPubMed ID15184548
Primary Source Matsunaga F. et al., Identification of short 'eukaryotic' Okazaki fragments synthesized from a prokaryotic replication origin. EMBO Rep. 2003 Feb4(2):154-8.PubMed ID12612604
Comments "Subsequently, the lagging strand polymerase transits from the 3'-OH terminus of the just completed Okazaki fragment to the new primer terminus, resuming DNA synthesis through 1000–2000 nt in E. coli and around 100 nt in P. abyssi (primary source)." See Lynch et al., 2015 doi: 10.1073/pnas.1514974112 link Supplementary Materials p.1 right column bottom paragraph:"For most prokaryotes, there is just a single origin of replication (ORI) per genome, and mean Okazaki fragment lengths are on the order of 1 to 2 kb [refs 12-14] as there are many thousands of such fragments per genome, it is clear that the vast majority of the costs associated with primer preparation and ligation are associated with lagging strands."
Entered by Uri M
ID 110937