Length of RNA primer synthesized every cycle of Okazaki fragments

Range 10 - 12 nts
Organism Unspecified
Reference Rocha EP. The replication-related organization of bacterial genomes. Microbiology. 2004 Jun150(Pt 6):1609-27. p.1611 left column top paragraphPubMed ID15184548
Primary Source Kitani, T., Yoda, K., Ogawa, T. & Okazaki, T. (1985). Evidence that discontinuous DNA replication in Escherichia coli is primed by approximately 10 to 12 residues of RNA starting with a purine. J Mol Biol 184 , 45–52PubMed ID2411935
Comments "Because DNA polymerization occurs in the 5'?3' direction, one strand is replicated continuously – the leading strand – whereas the other strand is replicated in discrete steps – the lagging strand – through the use of the Okazaki fragments. The cycle of the synthesis of Okazaki fragments starts by the synthesis of a new 10–12 nt RNA primer by a primase (primary source)." See Fijalkowska et al., 2012 PMID 22404288 p.1114 left column 2nd paragraph: "...the length of RNA primers is 10–20 nucleotides (Zechner et al., 1992a, b)."
Entered by Uri M
ID 110936