Diffusion coefficient of mRNA-protein complexes (mRNPs) in the nucleus of U2OS osteosarcoma cell measured by SPT [Single-particle tracking] at 37°C

Value 0.04 µm^2/sec Range: 0.01 - 0.09 µm^2/sec
Organism Human Homo sapiens
Reference Shav-Tal Y et al., Dynamics of single mRNPs in nuclei of living cells. Science. 2004 Jun 18 304(5678):1797-800. p.1797 right column top paragraphPubMed ID15205532
Comments p.1797 right column top paragraph:"Movements of nuclear mRNPs were followed by sequential imaging of living cells, and the obtained signal was enhanced by deconvolution (Fig. 1, A to C). Live cells at early times after induction (up to 30 min) presenting a low concentration of mRNPs were used to continuously track individual particles and study their motion (Fig. 1D). Single-particle tracking (SPT) was performed on mRNPs that remained in focus for a minimum of eight consecutive frames (>3 s) (Fig. 1, E to H) [movies S6 to S10 (ref 5)] (ref 3). Total distances traveled for tracked mRNPs were from 2 to 10 µm (mean 5 µm), and mean velocities ranged from 0.3 to 0.8 µm/sec (fig. S3A). These mRNPs displayed a diffusive pattern of movement (Fig. 1E) following a simple diffusion model <r^2> = 4Dt (Fig. 1J) (ref 3). The diffusion coefficients at 37°C (D37) ranged from 0.01 to 0.09 µm2/sec (mean 0.04 µm2/sec) (Fig. 1K)." See BNID 111851, 111853
Entered by Uri M
ID 111852