Calculated diffusion coefficient of RNP [ribonucleoprotein particles] complexes containing GFP–PABP2 [poly(A)-binding protein II]

Value 0.6 µm^2/sec Range: ±0.03 µm^2/sec
Organism Human Homo sapiens
Reference Calapez A et al., The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive. J Cell Biol. 2002 Dec 9 159(5):795-805. p.802 left column 2nd paragraphPubMed ID12473688
Method p.801 right column bottom paragraph:"In this work, [investigators] analyzed the mobility of GFP-tagged RNP complexes in the nucleus of living HeLa cells using photobleaching techniques. Bulk RNPs containing poly(A) RNA were visualized with GFP–PABP2. In addition, GFP– TAP was used to tag export-competent mRNP complexes." TAP is an export factor
Comments "Here [investigators] estimated a diffusion coefficient of 0.6 ± 0.03 µm^2/sec for RNP complexes containing GFP–PABP2 in the nucleus. A very similar value (0.6 ± 0.1µm^2/sec) was reported by Politz et al. (BNID 111845) using fluorescein-labeled oligo(dT) hybridized to nuclear poly(A) RNA."
Entered by Uri M
ID 111849