mRNA diffusion coefficient in U2OS and HEK293 cells

Value 1.06e-3 µm^2/sec
Organism Human Homo sapiens
Reference Yan X, Hoek TA, Vale RD, Tanenbaum ME. Dynamics of Translation of Single mRNA Molecules In Vivo. Cell. 2016 May 5 165(4):976-89. doi: 10.1016/j.cell.2016.04.034. p.979 left column 3rd paragraphPubMed ID27153498
Method P.987 left column 4th paragraph: "In summary, here [investigators] have developed an imaging method that enables the measurement of ribosome initiation and translocation rates on single mRNA molecules in live cells." P.987 left column bottom paragraph: "U2OS [human osteosarcoma U2OS cell line] and HEK293 [Human Embryonic Kidney 293 cell] cells were grown in DMEM/5% with Pen/Strep."
Comments P.979 left column 3rd paragraph: "Because single mRNAs were tethered to the plasma membrane through multiple PP7 [bacteriophage coat protein] molecules and thus through many CAAX membrane insertion domains, the 2D diffusion of mRNAs was extremely slow (1.06 × 10^−3 μm^2/s, n = 211 mRNAs). This slow diffusion made it possible to track individual mRNAs and their associated translation sites for extended periods of time (mean tracking time >1 hr) (Figure S1C)."
Entered by Uri M
ID 112742