| Range |
nucleolar RNA ~20%: nucleoplasmic RNA 80% %
|
| Organism |
Human Homo sapiens |
| Reference |
Jackson DA, Pombo A, Iborra F. The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 2000 Feb14(2):242-54. p.243 right column 3rd paragraphPubMed ID10657981
|
| Primary Source |
Soeiro, R., Vaughan, M. H., Warner, J. R., and Darnell, J. E., Jr. (1968) The turnover of nuclear DNA-like RNA in HeLa cells. J. Cell Biol. 39, 112–118PubMed ID5678443
|
| Method |
"...analysis performed on HeLa
cells, labeled for 10 min with orthophosphate-32P" |
| Comments |
"A complementary analysis performed on HeLa
cells, labeled for 10 min with orthophosphate-32P,
confirmed that nucleolar and nucleoplasmic RNA
accounts for ~20 and 80% of synthesis, respectively
(primary source). However, in view of their unusually high level
of nRNA turnover (31), it is worth considering if
cells adapted for continuous culture have developed
atypical patterns of RNA metabolism." |
| Entered by |
Uri M |
| ID |
111163 |