| Range |
~30 %
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Jackson DA, Pombo A, Iborra F. The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 2000 Feb14(2):242-54. p.243 right column 3rd paragraphPubMed ID10657981
|
| Primary Source |
Puvion E, Moyne G. Intranuclear migration of newly synthesized extranucleolar ribonucleoproteins. A high resolution quantitative autoradiographical and cytochemical study. Exp Cell Res. 1978 Aug115(1):79-88.PubMed ID680016
|
| Method |
"...the distribution of nRNA has
been analyzed in freshly isolated rat hepatocytes that
were treated with hydrocortisone, labeled for 5 min
with [3H]uridine, and subsequently grown in medium
with excess uridine..." |
| Comments |
"...in view of their unusually high level
of nRNA turnover (Harris (1963) Nuclear ribonucleic acid. Prog. Nucleic Acids
Res. 2, 19–59), it is worth considering if
cells adapted for continuous culture have developed
atypical patterns of RNA metabolism. In fact, studies
on freshly isolated cells indicate that this is not the
case. For example, the distribution of nRNA has
been analyzed in freshly isolated rat hepatocytes that
were treated with hydrocortisone, labeled for 5 min
with [3H]uridine, and subsequently grown in medium
with excess uridine (primary source). In these cells, nucleolar
transcripts account for ~30% of RNA synthesis,
while the remainder, in the nucleoplasm, behave like
pol II transcripts in immortalized cells." |
| Entered by |
Uri M |
| ID |
111164 |