| Range |
Table - link doubling time/hour
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Fan J, Kamphorst JJ, Mathew R, Chung MK, White E, Shlomi T, Rabinowitz JD. Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia. Mol Syst Biol. 2013 Dec 3 9: 712. doi: 10.1038/msb.2013.65. Supplementary information p.8 table 1PubMed ID24301801
|
| Method |
"To study ATP production routes, [researchers] used Bax-/-, Bak-/-
murine renal epithelial cells immortalized by expression of
adenovirus E1A and dominant-negative p53 (Degenhardt et al,
2002) (iBMK cells). Isogenic cell lines were generated by
transfecting cells with vector expressing either oncogenic
H-RasV12G or myr-Akt (Degenhardt et al, 2006 Degenhardt and
White, 2006). Note that activation of these specific genes may
not result in identical metabolic consequences to that of other
related family members that are frequently mutated in cancer
(e.g., K-Ras, PI3KCA)." |
| Comments |
"Introduction of either oncogene did not substantially impact cellular growth in vitro (Supplementary
Table 1), but greatly enhances tumorigenicity in vivo, as
evident by faster allograft growth, with the effect of Ras yet
stronger than that of Akt (Degenhardt and White, 2006)." iBMK=Immortalized baby mouse kidney epithelial cells |
| Entered by |
Uri M |
| ID |
110686 |