Metabolic flux distributions of parental, Ras, Akt cells in normoxia and parental iBMK cells in hypoxia

Range Table - link nmole/hour/μl cells
Organism Mammalian tissue culture cell
Reference Fan J, Kamphorst JJ, Mathew R, Chung MK, White E, Shlomi T, Rabinowitz JD. Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia. Mol Syst Biol. 2013 Dec 3 9: 712. doi: 10.1038/msb.2013.65. Supplementary information p.9 table 3PubMed ID24301801
Comments "To infer intracellular metabolic fluxes, [researchers] constructed a metabolic network model of glycolysis and TCA cycle (Supplementary Table 3) and applied metabolic flux analysis (MFA) to identify a flux distribution that optimally fits the following experimental data sets (Supplementary Figures 1–4 and Supplementary Table 5 BNID 110690): (i) measured uptake and secretion rates of glucose, glutamine, lactate, pyruvate, and alanine (ii) measured oxygen consumption rate used by oxidative phosphorylation (iii) steady-state isotopic labeling pattern of pyruvate, a-ketoglutarate, citrate, malate, and cytosolic acetyl-CoA, where the labeling pattern of cytosolic acetyl-CoA was inferred based on the steady-state labeling pattern of fatty acids via isotopomer spectral analysis (ISA)(Kharroubi et al, 1992) (iv) consumption of metabolic intermediates for biomass production based on measured growth rate and biomass component contents. Specifically, [researchers] measured the DNA, RNA, and protein contents to be equal to 11, 14, and 88 µg/µl cells, respectively, in iBMK cells. Cellular demands for acetyl-CoA based on steady-state fatty acid concentrations, cellular growth rate, and fatty acid uptake rates are shown in Supplementary Table 5 and (v) measured flux in the serine biosynthesis pathway." iBMK=Immortalized baby mouse kidney epithelial cells
Entered by Uri M
ID 110689