Fraction of reducing power, of the total NADH/FADH2 production in iBMK cell

Range oxidation of glutamine 60, oxidation of glucose 30, oxidation of acetyl-CoA 10 %
Organism Mammalian tissue culture cell
Reference Fan J, Kamphorst JJ, Mathew R, Chung MK, White E, Shlomi T, Rabinowitz JD. Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia. Mol Syst Biol. 2013 Dec 3 9: 712. doi: 10.1038/msb.2013.65. p.3 right columnPubMed ID24301801
Method "To study ATP production routes, [researchers] used Bax-/-, Bak-/- murine renal epithelial cells immortalized by expression of adenovirus E1A and dominant-negative p53 (Degenhardt et al, 2002) (iBMK cells)."
Comments "Tracking the source of reducing power, [researchers] found that oxidation of glutamine, glucose, and acetyl-CoA derived from other sources (such as unlabeled fatty acids or amino acids) contributes 60, 30, and 10% of the total NADH/FADH2 production, respectively." iBMK=Immortalized baby mouse kidney epithelial cells
Entered by Uri M
ID 110685