| Range |
3 to >90 min
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Bregman A, Avraham-Kelbert M, Barkai O, Duek L, Guterman A, Choder M. Promoter elements regulate cytoplasmic mRNA decay. Cell. 2011 Dec 23 147(7):1473-83. doi: 10.1016/j.cell.2011.12.005. p.1473 right column 2nd paragraphPubMed ID22196725
|
| Primary Source |
Wang, Y., Liu, C.L., Storey, J.D., Tibshirani, R.J., Herschlag, D., and Brown, P.O. (2002). Precision and functional specificity in mRNA decay. Proc. Natl. Acad. Sci. USA 99, 5860–5865.PubMed ID11972065
|
| Method |
(primary source abstract:) "By using DNA microarrays, [researchers] precisely measured the decay of each yeast mRNA, after thermal inactivation of a temperature-sensitive RNA polymerase II." |
| Comments |
"Two major cytoplasmic decay pathways exist.
Both are initiated by shortening of the mRNA poly(A) tail. The
mRNA can then be exonucleolytically degraded by the exosome
from 3' to 5' or by the Xrn1p exonuclease from 5' to 3'. The latter
pathway involves removal of the mRNA 5' cap (m(7)GpppN)
(Decker and Parker, 1993), which is a prerequisite stage for
Xrn1p activity (Coller and Parker, 2004 Larimer et al., 1992
Parker and Song, 2004). Although most yeast mRNAs are
degraded by either or both of these pathways, the half-lives of
specific mRNAs vary widely, ranging from 3 min to more than
90 min (primary source)." |
| Entered by |
Uri M |
| ID |
110027 |