DNA chain growth in mouse fibroblasts line, L-929

Range 0.6 to 0.7 (3,600 to 4,200 bases/replication fork/min) μm/min
Organism Mouse Mus musculus
Reference Hand R, Tamm I. DNA replication: direction and rate of chain growth in mammalian cells. J Cell Biol. 1973 Aug58(2):410-8. p.415 left column 2nd paragraph and p.417 left column 2nd paragraphPubMed ID4269686
Method "[Researchers] have described previously the method used for quantitation of rate of DNA chain growth (Hand and Tamm, 1972). This was modified in the present experiments only by the use of larger doses of radioactivity ."
Comments "The data are shown in the upper graph in Fig. 4. Control cells showed a rate of chain elongation of 0.6-0.7 µm/min (3600-4200 bases/replication fork/min), and they maintained this rate throughout the 2 h of the experiment."
Entered by Uri M
ID 109282