Cell surface area determinations in Staphylococcus aureus, J-774 mouse macrophages, and L-929 fibroblasts

Range Table - link
Organism Various
Reference Kehle T, Herzog V. A colloidal gold labeling technique for the direct determination of the surface area of eukaryotic cells. Eur J Cell Biol. 1989 Feb 48(1):19-26 p.22 table IPubMed ID2743991
Method Abstract: "[Investigators] have developed a colloidal gold labeling technique for the direct quantitation of the cell surface area. The method is based on coating the cell surface with [195Au] colloidal gold-protein complexes followed by morphometric determination of the labeling density (gold particles/µm^2 cell surface) and radiometric determination of the total number of gold particles bound per cell. The ratio of both values directly gives the cell surface area." P.22 left column bottom paragraph: "Surface area of Staph. Aureus: accuracy of the method: Staph. Aur. Cells were coated with [125I]IgG-Au 6 nm (Fig.2) The surface area of the cells was determined from the total number of IgG-Au bound per cell (radiometrical measurement) and the labeling density of the IgG-Au complexes (morphometric measurement). The ratio of these both values gave an average surface area of 4.0µm^2 per staph. Aur. Cell (Tab. I). The surface area alternatively calculated from the average diameter of the cells was 3.6µm^2. This value was within the standard deviation of the results obtained by the colloidal gold labeling technique (Tab. I)."
Comments Please see note beneath table
Entered by Uri M
ID 115567