Period of time that individual replication foci, where DNA is synthesized, last

Range ~60 min
Organism Mouse Mus musculus
Reference Conti C, Saccà B, Herrick J, Lalou C, Pommier Y, Bensimon A. Replication fork velocities at adjacent replication origins are coordinately modified during DNA replication in human cells. Mol Biol Cell. 2007 Aug18(8):3059-67. p.3062 left column bottom paragraphPubMed ID17522385
Primary Source Leonhardt, H., Rahn, H. P., Weinzierl, P., Sporbert, A., Cremer, T., Zink, D., and Cardoso, M. C. (2000). Dynamics of DNA replication factories in living cells. J. Cell Biol. 149, 271–280. p.277 right column top paragraphPubMed ID10769021
Method "C2C12 mouse myoblast cells were grown in DME supplemented with 20% FCS."
Comments "The molecular combing of pulse-labeled DNA enabled [researchers] to investigate whether replication factories adapt to the widely varying replicon sizes of somatic cells, given that the replication program is believed to be fixed before S-phase. For example, if replication fork velocity is ~1 kb/min, a 1-Mb replicon would take over 8h to replicate compared with 70min for a 140-kb replicon. Moreover, individual replication foci, where DNA is synthesized, last for a period of ~60 min (Leonhardt et al., 2000)."
Entered by Uri M
ID 111199