Median half-life of mRNA transcripts
| Value | 9 Hours |
|---|---|
| Organism | Mammalian tissue culture cell |
| Reference | Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.338 left column top paragraphPubMed ID21593866 |
| Method | Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives. |
| Comments | NIH3T3 (mouse fibroblast) cells. See BNID 106377 |
| Entered by | Uri M |
| ID | 106378 |