Number of methionine aminopeptidase1 (MAP1) in logarithmically growing yeast cell
| Value | 20000 Copies/cell |
|---|---|
| Organism | Budding yeast Saccharomyces cerevisiae |
| Reference | Raue U, Oellerer S, Rospert S. Association of protein biogenesis factors at the yeast ribosomal tunnel exit is affected by the translational status and nascent polypeptide sequence. J Biol Chem. 2007 Mar 16282(11):7809-16 Table - link PubMed ID17229726 |
| Method | Construction of Plasmids,Purification of His6-tagged Standard Proteins,Determination of Protein Concentrations,Antibodies and Immunoblotting Procedures,Quantification of Ribosomes and RPBs in Yeast Cells,In Vitro Transcription and Translation,Cross-linking of Nascent Polypeptides to RPBs,Purification of FLAG-tagged RNCs under Native Conditions,Generation of Non-translating and Translating Ribosomes |
| Entered by | Uri M |
| ID | 104306 |