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Quantification of Ribosome-associated Protein Biogenesis factors (RPBs) and ribosomes in a logarithmically growing yeast cell

Range	Table - link
Organism	Budding yeast Saccharomyces cerevisiae
Reference	Raue U, Oellerer S, Rospert S. Association of protein biogenesis factors at the yeast ribosomal tunnel exit is affected by the translational status and nascent polypeptide sequence. J Biol Chem. 2007 Mar 16 282(11):7809-16PubMed ID17229726
Method	Construction of Plasmids,Purification of His6-tagged Standard Proteins,Determination of Protein Concentrations,Antibodies and Immunoblotting Procedures,Quantification of Ribosomes and RPBs in Yeast Cells,In Vitro Transcription and Translation,Cross-linking of Nascent Polypeptides to RPBs,Purification of FLAG-tagged RNCs under Native Conditions,Generation of Non-translating and Translating Ribosomes
Entered by	Uri M
ID	104303