| Value |
30
Fold
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Matsuzaki H, Kassavetis GA, Geiduschek EP. Analysis of RNA chain elongation and termination by Saccharomyces cerevisiae RNA polymerase III. J Mol Biol. 1994 Jan 28235(4):1173-92.PubMed ID8308883
|
| Method |
In vitro transcription and transcript release. Autoradiograms were scanned with a laser densitometer and analyzed with the aid of a software package developed by AMBIS (san diego) |
| Comments |
RNA chain elongation through assembled transcription complexes was uneven but relatively rapid: at 20 degrees C with 1 mM NTPs, the fastest RNA chains elongated at an average rate of 29 nucleotides (nt)/second, and the median RNA chains elongated at 21 to 22 nt/second on average. At 0 degrees celsius rate decreased 30-fold |
| Entered by |
Uri M |
| ID |
103658 |