| Range |
10-30 min
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Ben-Tabou de-Leon S, Davidson EH. Modeling the dynamics of transcriptional gene regulatory networks for animal development. Dev Biol. 2009 Jan 15 325(2):317-28 p. 319 left column 1st paragraphPubMed ID19028486
|
| Primary Source |
1) Schröder HC, Friese U, Bachmann M, Zaubitzer T, Müller WE. Energy requirement and kinetics of transport of poly(A)-free histone mRNA compared to poly(A)-rich mRNA from isolated L-cell nuclei. Eur J Biochem. 1989 Apr 15 181(1):149-58. AND (2) Fuke H, Ohno M. Role of poly (A) tail as an identity element for mRNA nuclear export. Nucleic Acids Res. 2008 Feb36(3):1037-49PubMed ID2565812, 18096623
|
| Method |
[Primary source (1)] In vitro system shown to be an adequate model for in vivo mRNA transport. Isolated nuclei of mouse lymphoma L5178y cells. |
| Comments |
[Primary source (1) p. 153 left column top paragraph] Dot-blot analysis of the RNA in the postnuclear supernatants,
after different incubation periods, revealed (Fig. 3A)
that the ATP-promoted efflux of histone mRNA is already
complete after 5 min, while the amounts of transported actin
mRNA continually increased during the whole incubation
period (30 min), reaching maximal rates during the first
10 min. See BNID 105650 |
| Entered by |
Uri M |
| ID |
105622 |