5 - 10 min
|Mammalian tissue culture cell
|Upadhyaya A, Sheetz MP. Tension in tubulovesicular networks of Golgi and endoplasmic reticulum membranes. Biophys J. 2004 May86(5):2923-8. p.2923 right column top paragraphPubMed ID15111408
|Sciaky N et al., Golgi tubule traffic and the effects of brefeldin A visualized in living cells. J Cell Biol. 1997 Dec 1 139(5):1137-55.PubMed ID9382862
|Primary source abstract: "Here, [investigators] use green fluorescent protein-Golgi protein chimeras to study Golgi morphology in vivo."
|P.2923 right column top paragraph: "Although intact Golgi tubules can extend through the cytoplasm for 5–10 min normally, the fusion of Golgi tubules with ER causes the Golgi contents to mix into the ER within 15–30 s (primary source). Sciaky and co-workers (primary source) interpret their results as an indication of tension-driven flow between the two
membrane compartments. The ER is thought to provide a lower energy environment for membrane protein and lipid than the Golgi system, and flow rather than diffusion could cause mixing of components. The first step in verifying this hypothesis is to measure the tensions in the ER and Golgi
tubules directly." Primary source abstract: "A tubule network extending throughout the cytoplasm was quickly generated and persisted for 5-10 min until rapidly emptying Golgi contents into the ER within 15-30 s." Primary source investigated HeLa and CHO cells