Coefficient of determination (R^2) betweeen mRNA and protein copy number

Value 0.41 Unitless
Organism Mammalian tissue culture cell
Reference Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.339 right column 3rd paragraph and p. 338 fig.3dPubMed ID21593866
Method Stable isotope labelling by amino acids in cell culture (SILAC). Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives.
Comments NIH3T3 (mouse fibroblast) cells. Assuming the absence of technical and biological noise, this means that ~40% of the variance in protein levels is explained by mRNA levels—considerably more than previously thought.
Entered by Uri M
ID 106385