||Mammalian tissue culture cell
||Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.339 right column 3rd paragraph and p. 338 fig.3dPubMed ID21593866
||Stable isotope labelling by amino acids in cell culture (SILAC). Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives.
||NIH3T3 (mouse fibroblast) cells. Assuming the absence of technical and biological noise, this means
that ~40% of the variance in protein levels is explained by mRNA
levels—considerably more than previously thought.