| Comments |
P.1647 right column bottom paragraph & p.1649 left column top paragraph : "The ensemble of sequences in this study provides an overarching view of the mammal gut microbiota. [Investigators] detected members of 17 phyla (divisions) of Bacteria (primary source 10). The majority of sequences belong to the Firmicutes [65.7% of 19,548 classified sequences (primary source 10)] and to the Bacteroidetes (16.3%) these phyla were previously shown to constitute the majority of sampled human (and mouse) gut-associated phylotypes (primary sources 10, 20). The other phyla represented were the Proteobacteria (8.8% of all sequences collected 85% in the Gamma subdivision), Actinobacteria (4.7%), Verrucomicrobia (2.2%), Fusobacteria (0.67%), Spirochaetes (0.46%), DSS1 (0.35%), Fibrobacteres (0.13%), TM7 (0.13%), deep-rooting Cyanobacteria [0.10% these are not chloroplasts (primary source 20)], Planctomycetes (0.08%), Deferribacteres (0.05%), Lentisphaerae (0.04%), and Chloroflexi, SR1, and Deinoccus-Thermus (all 0.005%). [They] were unable to assign 1985 16S rRNA gene sequences that passed a chimera-checking algorithm (ref 21) to known phyla on the basis of BLAST searches against the Greengenes database (ref 22) and the Ribosomal Database Project taxonomy annotations (ref 23). Of the phyla that were detected, only Firmicutes were found in all samples (fig. S1). However, each mammalian host harbored OTUs [operational
taxonomic units](96% sequence identity) not observed in any other sample (at this level of sampling, on average, 56% and 62% of OTUs were unique within a sample and species, respectively table S1)." |