Bacterial content of mammalian gut derived from bacterial 16S ribosomal RNA gene sequences

Range Firmicutes 65.7%: Bacteroidetes 16.3%: Proteobacteria 8.8%: see Comments section for the rest %
Organism bacteria
Reference Ley RE et al., Evolution of mammals and their gut microbes. Science. 2008 Jun 20 320(5883):1647-51. doi: 10.1126/science.1155725. P.1647 right column bottom paragraph & p.1649 left column top paragraphPubMed ID18497261
Primary Source [10] See supporting material on Science Online link [20] Ley RE, Bäckhed F, Turnbaugh P, Lozupone CA, Knight RD, Gordon JI. Obesity alters gut microbial ecology. Proc Natl Acad Sci U S A. 2005 Aug 2 102(31):11070-5. DOI: 10.1073/pnas.0504978102 PubMed ID16033867
Method Abstract: "To understand the coevolution of the mammals and their indigenous microbial communities, [investigators] conducted a network-based analysis of bacterial 16S ribosomal RNA gene sequences from the fecal microbiota of humans and 59 other mammalian species living in two zoos and in the wild."
Comments P.1647 right column bottom paragraph & p.1649 left column top paragraph : "The ensemble of sequences in this study provides an overarching view of the mammal gut microbiota. [Investigators] detected members of 17 phyla (divisions) of Bacteria (primary source 10). The majority of sequences belong to the Firmicutes [65.7% of 19,548 classified sequences (primary source 10)] and to the Bacteroidetes (16.3%) these phyla were previously shown to constitute the majority of sampled human (and mouse) gut-associated phylotypes (primary sources 10, 20). The other phyla represented were the Proteobacteria (8.8% of all sequences collected 85% in the Gamma subdivision), Actinobacteria (4.7%), Verrucomicrobia (2.2%), Fusobacteria (0.67%), Spirochaetes (0.46%), DSS1 (0.35%), Fibrobacteres (0.13%), TM7 (0.13%), deep-rooting Cyanobacteria [0.10% these are not chloroplasts (primary source 20)], Planctomycetes (0.08%), Deferribacteres (0.05%), Lentisphaerae (0.04%), and Chloroflexi, SR1, and Deinoccus-Thermus (all 0.005%). [They] were unable to assign 1985 16S rRNA gene sequences that passed a chimera-checking algorithm (ref 21) to known phyla on the basis of BLAST searches against the Greengenes database (ref 22) and the Ribosomal Database Project taxonomy annotations (ref 23). Of the phyla that were detected, only Firmicutes were found in all samples (fig. S1). However, each mammalian host harbored OTUs [operational taxonomic units](96% sequence identity) not observed in any other sample (at this level of sampling, on average, 56% and 62% of OTUs were unique within a sample and species, respectively table S1)."
Entered by Uri M
ID 112854