ORF, predicted ORF product molecular size, isoelectric point & apparent molecular mass of NaChBac [bacterial ion channel]

Range ORF 274 amino acids: predicted 31 kDa: calculated isoelectric point 9.35: apparent ~34kDa
Organism Bacteria Bacillus halodurans
Reference Ren D et al., A prokaryotic voltage-gated sodium channel. Science. 2001 Dec 14 294(5550):2372-5. p.2372 right column bottom paragraph to p.2373 left columnPubMed ID11743207
Method P.2375 middle column note 10:"NaChBac was cloned from Bacillus halodurans C-125 DNA by the polymerase chain reaction (PCR) using Pfu polymerase and primers designed according to the deposited sequence (GenBank accession number BAB05220). The deduced sequence is identical to the deposited sequence except for two differences: nucleotide 35 (T in [investigators'] clone versus C in the deposited sequence no change in amino acid) and nucleotide 818 (T versus C Leu to Ser amino acid change). There is a large deletion (>1 kb) in the deposited sequence immediately after the ORF. Multiple clones from different amplification reactions were sequenced to verify [investigators'] cDNA. Errors in the deposited C-125 sequence probably result from the shotgun method of sequence assembly."
Comments P.2372 right column bottom paragraph to p.2373 left column:"Isolation and sequencing of the gene encoding NaChBac (see 'Method' section above) revealed an open reading frame (ORF) of 274 amino acids with a predicted molecular size of 31 kD and a calculated isoelectric point of 9.35 (Fig. 1A). NaChBac expressed in bacteria migrated during SDS–polyacrylamide gel electrophoresis with an apparent molecular mass of ∼34 kD (Fig. 1B)."
Entered by Uri M
ID 112274