Fraction of total water in bacterial pellets that may be intercellular water

Range >60 %
Organism bacteria
Reference Bratbak G, Dundas I. Bacterial dry matter content and biomass estimations. Appl Environ Microbiol. 1984 Oct48(4):755-7. p.755 left column top paragraphPubMed ID6508285
Primary Source [4] Buckmire FL, MacLeod RA. Penetrability of a marine pseudomonad by inulin, sucrose, and glycerol and its relation to the mechanism of lysis. Can J Microbiol. 1970 Feb16(2):75-81. [17] Sprott GD, Drozdowski JP, Martin EL, MacLeod RA. Kinetics of Naplus-dependent amino acid transport using cells and membrane vesicles of a marine pseudomonad. Can J Microbiol. 1975 Jan21(1):43-50. [19] Thompson J, MacLeod RA. Functions of Na+ and K+ in the active transport of -aminoisobutyric acid in a marine pseudomonad. J Biol Chem. 1971 Jun 25 246(12):4066-74.PubMed ID5417664, 1116038, 5561475
Comments P.755 left column top paragraph: "Percent dry matter of bacterial cells is usually determined by weighing a bacterial pellet before and after drying to constant weight. Available information on the water content in bacterial pellets, however, suggests that >60% of the total water in the pellets may be intercellular water (primary sources)." See also p.756 left column top paragraph: "The percent intercellular water of [investigators’] pellets ranged from 32 to 91%, which, taking into account [their] rather low centrifugation speed and the natural variability between bacterial types, is comparable to the range of 40 to 69% intercellular water reported by others (refs 4, 17, 19)."
Entered by Uri M
ID 114792