Length of flanking homologous regions required for gene targeting through homologous recombination in mouse embryonic stem (ES) cells

Value >=2 kb Range: The recommended total length of two flanking homologous sequences is 6-10 kb kb
Organism Mouse Mus musculus
Reference Vega biolab-Partner for gene targeting 3701 Market Street, Philadelphia, PA 19104 link
Comments "FAQs: Q. What is the required minimum length of homologous region? A. minimum of 2 kb on each homologous arm is recommended. Total of 6-10 kb homologous genomic DNA should give reasonably satisfactory result. If your gene targeting facility has different requirements, [researchers] can make the design accordingly to accommodate the requirements." Regions of homology between the targeting vector and the target locus should be as large as possible to maximize gene targeting efficiency.
Entered by Irina
ID 101987