Mean length (L) and width (W) of each strain at the beginning (T0) and at the end (T4) of starvation experiments

Range Table - link µm
Organism bacteria
Reference Marc Troussellier, Marc Bouvy, Claude courties, Christine dupuy, Variation of carbon content among bacterial species under starvation condition, Aquatic microbial ecology, Vol 13 :113-119. 1997 link p.117 table 1
Method P.114 right column 2nd & 3rd paragraphs: "At the same time, a subsample was filtered onto a black Nuclepore filter (0.2µm pore size) which was stored at 5˚C in the dark, and examined, within 48 h, with an epifluorescence microscope (Olympus BH with a 100×UVFL iris objective) to determine size of DAPI stained bacteria. Photographs (Kodak Tmax ASA 400) were taken from 10 randomly selected fields, then projected onto a digitizer tablet (final magnification, ×5000). Dimensions of 50 cells per sample were measured with a computer-assisted digitizer. Cell volume (V) was calculated assuming that the shape of bacteria was spherical or cylindrical with hemispheric ends: V=(πw^2/4)(l-w/3), where w is the width and l is the length of the cell (Bratbak 1985). Calibration was performed with microphotographs of a slide micrometer (Zeiss) and microspheres (Polysciences, Warrington, PA) of 0.51 and 0.75µm diameter."
Comments P.114 right column 4th paragraph: "Results. Changes in measured variables and computed conversion factors for marine and non-marine strains are reported in Figs. 1 & 2, respectively. Cell abundances showed either a slow but significant decreasing trend with time (all marine strains, Aeromonas hydrophyla, and Enterobacter cloacae), or stability (the other non-marine strains). In all incubations the average POC [particulate organic carbon] and cell volume values also showed a significant decrease with time. The ratio between cell length and width decreased with decreasing average cell volume, with the exception of Vibrio fischeri cells which have an initial low ratio (Table 1). Thus, cells tended to be more coccoid during incubation."
Entered by Uri M
ID 112932