Volume of cells at sub-seafloor deposits at Landsort Deep, Baltic Sea

Range filtered 0.68µm^3: non-filtered 0.76 µm^3
Organism Bacteria Escherichia coli
Reference Braun S et al., Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea. Front Microbiol. 2016 Aug 31 7: 1375. doi: 10.3389/fmicb.2016.01375 p.4 right column top paragraphPubMed ID27630628
Method Abstract: "To determine their shape and volume, cells were separated from the sediment matrix by multi-layer density centrifugation and visualized via epifluorescence microscopy (FM) and scanning electron microscopy (SEM). Total cell-carbon was calculated from amino acid-carbon, which was analyzed by high-performance liquid chromatography (HPLC) after cells had been purified by fluorescence-activated cell sorting (FACS)." P.4 left column bottom paragraph: "To obtain a correction factor to account for volume shrinkage from the filtration of cells onto filter membranes, [investigators] used fixed (2% PFA [paraformaldehyde] for 6 h at 4°C) E. coli and M. luteus cells"
Comments P.4 right column top paragraph: "For example, the mean cell volume of filtered E. coli cells was 0.68 μm^3, while that of non-filtered E. coli cells was 0.76 μm^3. The cell volumes of filtered elongated cells (rods and filaments) from the sediment samples were therefore corrected by the factor 0.76/0.68 = 1.13 (Table 1)."
Entered by Uri M
ID 115322