Carbon content per cell (Cc), mean & for each strain

Value 26.02 fg C/cell Range: ±1.08 Table - link fg C/cell
Organism bacteria
Reference Marc Troussellier, Marc Bouvy, Claude courties, Christine dupuy, Variation of carbon content among bacterial species under starvation condition, Aquatic microbial ecology, Vol 13 :113-119. 1997 link abstract & p.117 table 2
Method P.114 left column bottom paragraph: "Particulate organic carbon (POC) determination: At each sampling time, 3 replicate samples (50 ml) were withdrawn and filtered in a clean acid-washed and rinsed glass filter unit (Millipore) through 2 stacked precombusted (450°C ≥4 h) GF/F glass fiber filters (Whatman, 25 mm). Filters were dried at 60°C for >24 h and were assayed on a Leco CHN analyzer. At the beginning of the experiment, three 50 ml samples of suspension medium (ASW [sterile artificial seawater] and PW [sterile physiological water]) were filtered on 2 stacked precombusted GF/F filters to check for carbon contribution of suspension medium. At each time, 2 blank precombusted GF/F filters (no water filtered) were assayed. These control filters contained no measurable amounts of carbon. On the other hand, bacterial abundance (see hereafter) in each filtrate was estimated, showing that the 2 stacked filters retained at least 99% of bacterial cells in samples (data not shown)."
Comments Abstract: "To explore the variability of bacterial biomass conversion factors in aquatic environments, 5 marine and 5 non-marine bacterial strains were exposed to nutrient deprivation in artificial seawater and physiological (NaCl, 9 g/L) water, respectively. Carbon content per cell (Cc) and per volume (Cv) were estimated from total cell number, particulate organic carbon and cell volume (V) measurements after 0, 7, 15 and 28 d of starvation. While Cc appeared to be rather constant (26.02±1.08fg C/cell, mean±standard error, n = 50) during starvation, Cv showed a significant increase." P.114 right column bottom paragraph: "The small coefficient of variation associated with mean Cc [Carbon content per cell] value of each strain confirms the fairly constant value of their cellular carbon density (Table 2). While significant differences among Cc values of the different strains were detected by a 1-way ANOVA (Fvalue = 9.687 p < 0.0001), there was only a factor of 2.3 between the lowest (14.51, Vibrio campbellii) and the highest (33.45, V. fischeri) mean Cc values."
Entered by Uri M
ID 112933