Speed of NHDF [normal human dermal fibroblast] mesenchymal cell

Value 0.234 µm/min Range: ±0.09 µm/min
Organism Human Homo sapiens
Reference Liu YJ et al., Confinement and low adhesion induce fast amoeboid migration of slow mesenchymal cells. Cell. 2015 Feb 12 160(4):659-72. doi: 10.1016/j.cell.2015.01.007. p.663 left column 3rd paragraphPubMed ID25679760
Method P.663 left column 2nd paragraph:"To test whether this microenvironment-dependent switch in the mode of migration was restricted to NHDF cells, [researchers] investigated the migration behavior of 20 other cell types confined by fully non-adhesive surfaces (100% pLL-PEG). [They] chose a sample of cell types covering a large range of migratory and non-migratory cells, including normal and transformed epithelial cells (pre- and post-EMT [epithelial to mesenchymal transition]), normal and transformed mesenchymal cells, leukocytes (typical amoeboid cells), and muscle cell precursors."
Comments P.663 left column 3rd paragraph:"For all cell types, with the exception of normal epithelial cells, [researchers] found a sub-fraction of spontaneously polarizing cells moving fast with an amoeboid-like morphology (Figures 4A and 4B). Unexpectedly, [they] found two distinct types of fast-migrating cells (Figures 4A and S4A–S4C). The first type, which [they] named A1, was similar to what was observed with NHDF cells: a round cell body and a small leading edge (Movie S3). The second, which [they] named A2, had an elongated ellipsoid cell body with a large uropod and resembled a migrating neutrophil (Figures S4A and S4B Movie S3). Globally, A2 cells moved significantly faster (5.3 ± 1.5 μm/min) than A1 cells (1.7 ± 0.4 μm/min) and they both moved much faster than mesenchymal controls (0.234 ± 0.09 μm/min) (see Figures 4C and 4D)."
Entered by Uri M
ID 112237