Speed of A1 fast-migrating cells

Value 14.63 µm/min Range: ±0.9 µm/min
Organism Human Homo sapiens
Reference Liu YJ et al., Confinement and low adhesion induce fast amoeboid migration of slow mesenchymal cells. Cell. 2015 Feb 12 160(4):659-72. doi: 10.1016/j.cell.2015.01.007. p.665 right column 2nd paragraphPubMed ID25679760
Method P.665 right column top paragraph:"To characterize the migration mechanism of confined non-adhesive cells, [investigators] imaged focal adhesions, actin, and myosin II using TIRFM [total internal reflection microscopy]. On the fibronectin-coated surface, cells attached, spread, and formed focal adhesions and actin stress fibers containing myosin II (Figure 6A, upper panel Movie S6)." P.665 right column 2nd paragraph:"To assess whether A1 motility relied on a similar mechanism, [investigators] added 30 mM of the ROCK inhibitor Y27632."
Comments P.665 right column 2nd paragraph:"As with the A2 cells, [investigators] found that motile single A1 [for definition of A1 & A2 cells see comments section in BNID 112237] cells displayed a fast retrograde flow (14.63 ± 0.9 μm/min). Unlike the A2 cells, however, the flow was not global, from the front to the back of the cell. Instead, it was localized only in a small protruding region at the leading edge of the cell (Figures S6A and S6B Movie S8). The limited area of the retrograde flow might explain why these cells were slower than A2 cells."
Entered by Uri M
ID 112239