Selected fluxes and rates for the two scenarios included that were used for benchmarking

Range Table - link nmol/gFW/sec × C atoms
Organism Thale cress Arabidopsis thaliana
Reference Szecowka M et al., Metabolic fluxes in an illuminated Arabidopsis rosette. Plant Cell. 2013 Feb25(2):694-714. doi: 10.1105/tpc.112.106989. p.704 figure 1H & p.705 bottom paragraph caption to figure 1HPubMed ID23444331
Method P.695 right column 3rd paragraph:"In this study, [investigators] used a custom-designed labeling chamber to carry out short-term 13CO2 labeling of intact Arabidopsis rosettes under ambient steady state conditions. Several complementary analytical platforms were applied, including GC-TOF-MS [gas-chromatography-time-of-flight-mass spectrometry] and two recently developed liquid chromatography–tandem mass spectrometry (LC-MS/MS) platforms (Lunn et al., 2006 Arrivault et al., 2009), allowing quantitative determination and 13C enrichment calculation of 40 metabolites from the CBC [Calvin-Benson cycle], Suc and starch synthesis, glycolysis, amino acid and organic acid metabolism, as well as trehalose-6-phosphate (Tre6P) metabolism."
Comments P.705 left column 2nd paragraph:"[Investigators] first modeled fluxes assuming that the entire pool of each metabolite is involved in photosynthetic flux (Figure 5H, case All full results are presented in Supplemental Tables 5 and 6 online). The value of the variance-weighted mean squared error of the best fit was much larger than one (the variance-weighted mean square error of 8035 and 12 estimated parameters), suggesting unreliable goodness of fit. The estimated rate of RuBP oxygenation was about a third of the rate of carboxylation (3.72:10.70), and the rate of starch synthesis was ∼2.6 times higher than the rate of Suc synthesis (6.32:2.45). While the relation between carboxylation and oxygenation is close to expectations, the model seriously overestimates the rate of starch synthesis and underestimates the rate of Suc synthesis. The 95% confidence intervals for the estimated fluxes are given in Figure 5H interestingly, the data enforce a small and left-skewed estimate of the trehalose synthesis rate, causing it to fall out of its confidence interval." See note beneath table.
Entered by Uri M
ID 112096