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||Thale cress Arabidopsis thaliana
||Ma F, Jazmin LJ, Young JD, Allen DK. Isotopically nonstationary 13C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation. Proc Natl Acad Sci U S A. 2014 Nov 25 111(47):16967-72. doi: 10.1073/pnas.1319485111. p.16969 figure 3PubMed ID25368168
||Abstract: "[Investigators] performed in vivo isotopic labeling of Arabidopsis thaliana rosettes with (13)CO2 and estimated fluxes throughout leaf photosynthetic metabolism by INST-MFA [Isotopically nonstationary metabolic flux analysis]. Plants grown at 200 µmol m^(-2)s^(-1) light were compared with plants acclimated for 9 d at an irradiance of 500 µmol⋅m^(-2)⋅s^(-1)."
||P.16970 left column 2nd paragraph: "Model-determined fluxes were not constrained to a particular measurement but rather were based on nonlinear regression of numerous MID [mass isotopomer distributions] measurements and experimentally derived starch and CO2 net assimilation rates. Each model included 54 free flux parameters and required more than 1,000 ordinary differential equations (ODEs) to simulate the labeling time course of the measured MIDs (Fig. 3 and Figs. S2 and S3). Computing the sensitivities of all MIDs to the adjustable parameters required an additional ∼94,000 ODEs. The LL [Low Light] and HL-ACC [high light acclimated] models had sum-of-squared residuals (SSR) of 1,003 and 808, which were both accepted based on χ^2 tests with degrees of freedom equal to 1,139 and 1,019, respectively (Fig. 3)." See caption beneath figure