Amount and subcellular distribution of compounds

Range Table - link
Organism Thale cress Arabidopsis thaliana
Reference Szecowka M et al., Metabolic fluxes in an illuminated Arabidopsis rosette. Plant Cell. 2013 Feb25(2):694-714. doi: 10.1105/tpc.112.106989. p.699 table 1PubMed ID23444331
Method P.695 right column 3rd paragraph:"In this study, [investigators] used a custom-designed labeling chamber to carry out short-term 13CO2 labeling of intact Arabidopsis rosettes under ambient steady state conditions. Several complementary analytical platforms were applied, including GC-TOF-MS [gas-chromatography-time-of-flight-mass spectrometry] and two recently developed liquid chromatography–tandem mass spectrometry (LC-MS/MS) platforms (Lunn et al., 2006 Arrivault et al., 2009), allowing quantitative determination and 13C enrichment calculation of 40 metabolites from the CBC [Calvin-Benson cycle], Suc and starch synthesis, glycolysis, amino acid and organic acid metabolism, as well as trehalose-6-phosphate (Tre6P) metabolism."
Comments P.696 right column 3rd paragraph:"The absolute levels of the metabolites are shown in Table 1. There is a large dynamic range, even for metabolites in the same pathway (e.g., 11.7 and 173 nmol/g fresh weight [FW] for G1P and G6P, respectively), with the extremes being 0.55 (ADPG) and >1000 (Suc, Ser, Fru, and Glc) nmol/g FW." See note beneath table
Entered by Uri M
ID 112094